Western Blot & Electrical Tran
ID:36058 View:537次 |
Date Listed: | 2015-01-31 12:55:37 | Country: | United States»New York»New York |
Poster: | Candy | Company: | Creative Proteomics1 |
Location: | 45-16 Ramsey Road | Website: | Creative Proteomics1 |
Email: | candyton99@gmail.com | Mobile: | |
Fax: | MSN: |
"The transfer or ""blotting"" of electrophoretic separated proteins from the gel matrix to a membrane (typically nitrocellulose or PVDF) followed by subsequent antibody-based detection on the surface of the membrane is called Western Blot or Immunoblot. Creative Proteomics provides western blotting analysis for the detection of a specific target protein out of a complex protein mixture, e.g. tissue homogenate or cell extract, using highly selective and sensitive antibody-antigen interactions. The resulting data allow both qualitative and semi-quantitative analysis of the protein of interest.The Western Blot is a widely used analytical technique for the detection of specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure, or denatured proteins by the length of the polypeptide. The separated proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. Western Blot is widely used in the fields of molecular biology, biochemistry, immune genetics and other molecular biology disciplines. Blotting is the transfer of macromolecules on immobilizing membranes for specific and sensitive detection. In Western Blot analysis, the proteins of the sample are first separated in gel electrophoresis by isoelectric point (pI), molecular weight, electric charge, or a combination of these factors using various methods such as SDS-PAGE and IEF. Usually SDS-PAGE is employed for the separation, because all the proteins are solubilized and migrate in the same direction, and the epitopes are easier accessible due to the denaturing effect of SDS"
Please Note that this information was issued on BestB2B.com!
Insulation Panel,roof panels2024-01-29
EPS sandwich panel2024-01-29
Insulated Metal Panels Increas2024-01-29
Insulated Metal Roof Panel2024-01-29
Light Insulated Metal Wall Pa2024-01-29
Insulated Metal Wall Panels2024-01-29
Insulated sandwich panels / In2024-01-29
Trapezoidal Roof Panel / Wall2024-01-29
100G QSFP28 SWDM4 100m LC Opti2024-02-01
100G QSFP28 SR4 100M MPO Optic2024-02-01
200G QSFP56 SR4 100m MPO Optic2024-02-01
400G OSFP SR8 100m MPO Optical2024-02-01
400G QSFP-DD LR8 10KM LC Optic2024-02-01
400G QSFP-DD LR4 10KM LC Media2024-02-01
400G QSFP-DD 4x100G LR 10KM 2024-01-29